How to use ALL reads to calculate the RNAmetrics
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9.0 years ago
pengchy ▴ 450

Hi all,

I want to using all the reads to get the metrics from picard CollectRnaSeqMetrics. Is it ok to set METRIC_ACCUMULATION_LEVEL=ALL_READS? I want to know the value of the following metrics using all reads:

"MEDIAN_CV_COVERAGE"    "MEDIAN_5PRIME_BIAS"    "MEDIAN_3PRIME_BIAS"    "MEDIAN_5PRIME_TO_3PRIME_BIAS"

Thank you!
RNA-Seq picard • 1.4k views
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