Hi, Im ungraduate student. Please help me.
I want mapping-bam-file from sra-dataset from NCBI in order to analyze heterogeneiety of mouse ESC.
The dataset is generated from a paper below.(GSE60749)
Roshan M.Kumar et al. Deconstructing transcriptional heterogeneity in pluripotent stem cells. Nature(2014)
Please teach me how to get adapter sequence used in this experimentation
and what I should use for quality control(Prinseq?ShortRead?).
For example, I want to try GEO Sample GSM1486817 sra file.
Can anyone give me process of quality control of this sra?
I thank you for reading it through.
Any help will be appreciated.