Question: what is difference between sequence adapter and PCR primer?
2
gravatar for xiangwulu
3.8 years ago by
xiangwulu50
Ireland
xiangwulu50 wrote:

what are the differences between pcr primer and adapter? are they all artificial sequence added in sequence amplification process? and they can be both exist in raw sequence reads?

thanks.  

pcr adapter • 18k views
ADD COMMENTlink modified 3.8 years ago by Anima Mundi2.4k • written 3.8 years ago by xiangwulu50
4
gravatar for Anima Mundi
3.8 years ago by
Anima Mundi2.4k
Italy
Anima Mundi2.4k wrote:

Hello,

both PCR primers and sequence adaptors are artificial DNA oligonucleotides, generally of known sequence. The first are used to prime DNA replication reactions. Those used to amplify specific DNA sequences in vitro are called PCR primers. Sequence adaptors are any kind of short DNA sequence serving the scope of fishing a (generally unknown) DNA sequence of interest for various purposes; they are used in a variety of techniques, and sometimes can take part in a DNA replication step (e.g. in a 5'RACE). Depending on experimental protocols and following bioinformatics, both PCR primers and sequence adaptors can end up in raw sequence reads (for instance, this may occur if you are sequencing a DNA library).

ADD COMMENTlink modified 3.8 years ago • written 3.8 years ago by Anima Mundi2.4k

thank you               

ADD REPLYlink written 3.8 years ago by xiangwulu50
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