So I downloaded the ncbi blast suite and I'm trying to run the command line.
I have a text file with a protein sequence at ~/in.txt:
I used scp to copy the database files from my school [UCSC] server to my local drive in ~/blast_db
I ran the following command:
blastp -query ~/in.txt -out output_blast.txt -db /Users/Mu/blast_db/nr.32.psq
This is my error:
eduroam-16180339887:~ Mu$ blastp -db ~/blast_db/nr.32.psq -query ~/in.txt -out output_blast.txt BLAST Database error: No alias or index file found for protein database [/Users/Mu/blast_db/nr.32.psq] in search path [/Users/Mu::]
The files are binary for the database. I looked up the suffix on NCBI and .psq is what to use for protein database. How can I get this to work?