MiSeq - reference database for ITS
0
0
Entering edit mode
6.4 years ago
User 4014 ▴ 40

HI Folks,

 

I am a beginner learning to use MOTHUR for analysis of my fungal ITS1/2 datasets. I followed MOTHUR MiSeq SOP which relies on bacterial 16S until the step align.seqs. I understand that I need another reference database in this step, may I ask which reference database? And which command should I use in case I can't use align.seqs? I appreciate if you could recommend a step-by-step tutorial regarding Mothur and ITS analysis that I can learn from.

 

Thank you in advance and have a good day!

Vinn

 

 

amplicon sequencing MOTHUR ITS • 2.9k views
ADD COMMENT
0
Entering edit mode

Have you looked at this?

ADD REPLY
0
Entering edit mode

HI h.mon,

Thanks for the link. So I guess if I use UNITE for ITS2 I can just use the command classify.seqs?

ADD REPLY

Login before adding your answer.

Traffic: 2026 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6