Question: MiSeq - reference database for ITS
0
gravatar for User 4014
4.3 years ago by
User 401430
Sweden
User 401430 wrote:

HI Folks,

 

I am a beginner learning to use MOTHUR for analysis of my fungal ITS1/2 datasets. I followed MOTHUR MiSeq SOP which relies on bacterial 16S until the step align.seqs. I understand that I need another reference database in this step, may I ask which reference database? And which command should I use in case I can't use align.seqs? I appreciate if you could recommend a step-by-step tutorial regarding Mothur and ITS analysis that I can learn from.

 

Thank you in advance and have a good day!

Vinn

 

 

amplicon sequencing its mothur • 2.3k views
ADD COMMENTlink modified 4.3 years ago • written 4.3 years ago by User 401430

Have you looked at this?

ADD REPLYlink written 4.3 years ago by h.mon27k
0
gravatar for User 4014
4.3 years ago by
User 401430
Sweden
User 401430 wrote:

HI h.mon, 

Thanks for the link. So I guess if I use UNITE for ITS2 I can just use the command classify.seqs?

 

ADD COMMENTlink written 4.3 years ago by User 401430
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1581 users visited in the last hour