I'm writing my own short read read simulator (mostly for fun, I known I'm reinventing the wheel).
The 'beta' source code is available here: http://code.google.com/p/variationtoolkit/source/browse/trunk/src/shortreadsim.cpp
Here is my question:
- each short read has a length = 'short_ read_length'
- a gene has a length = 'segLength'
how many short-reads do I need to create to have an average coverage = 'N' for this gene ? (simple math ?)
Thanks Michael, I must be very tired... :-)