Hello to everyone,
I have output from RT-PCR (Applied Biosystems - DataAssist software). The output is an excel sheet containing CT values, Delta CT, and 2 ^ (-CT) for each sample.
For each gene I have a list of samples. For each sample I have three groups (control(or reference), Adenoma and Hyperplastic). For each sample I also have the CT value of target gene and selected control.
So each sample is categorized under one of these three groups (and values are available for target gene and selected control).
Now it's easy to compute "Delta CT" for each sample.
I just don't know how to compute "Delta Delta CT" for each group.
For example I have 9 controls, 14 Hyperplastic and 30 Adenoma. Now how "Delta Delta CT" should be computed and How a total fold change value should be computed for it?
* I know Data Assist software also does this for me, but I need to recompute it for some purpose.
Any help is highly appreciated.