I am a beginner in the bioinformatics and I have some questions.
When I read some papers regarding the mutational landscape, nearly all of them described the Ti Tv ratio in terms of mutation spectrum with some pretty figures. Therefore I would like to apply this statistics to my research but I couldn't find its definition in a easy way and how to calculate it. Also, I have read some articles that nowadays ti/tv ratio has little impact when it comes to analyzing exome sequencing.
Therefore, I would like to ask you the following questions:
1) Ti Tv ratio: how to calculate it? Is there a website or tools that get them in an automatic way? If not, what should I know before writing some codes by myself?
2) for exome sequencing, all the things are equally applied when analyzing whole genome sequencing data?