How to find out off target reads?
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7.6 years ago

Here is the summary of Ion Torrent run result. Valid reads is only 19% how to improve it? How to find reads outside target region?

Reads                   21481392
Mapped Reads            19442218
Mapped %                90.50725391
Valid Reads             19.67%
Total-Reads on Target   3823517
On Target               24.72%
Off Target Reads        15618701
iontorrent RNA-Seq Off-Target-Reads • 2.4k views
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Can't resist citing @BioMickWatson ...

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what the meaning of ur message?

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Oh, come on... I agree for Solid, but surely Ion Torrent is much better.

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