Question: Can we call a protein under undergone ChIP as ChIP'd protein? Is it formal?
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gravatar for Sukhi Singh
5.6 years ago by
Sukhi Singh10k
Netherlands
Sukhi Singh10k wrote:

More of a grammatical question, I am writing a report and was wondering, can we call a protein which was pulled down using ChIP (chromatin immunoprecipitation) as ChIP'd protein? I have seen some articles, tutorials and even papers using it.

Also, "pulling down/pulled down a protein" is it formal for writing purposes.

Have a good day!
 

sequencing ngs chip-seq chip • 1.1k views
ADD COMMENTlink modified 2.0 years ago by Biostar ♦♦ 20 • written 5.6 years ago by Sukhi Singh10k
1
gravatar for michael.law26
5.6 years ago by
michael.law2610 wrote:

I think you should say something like, "following crosslinking, immunoprecipitations were performed using antibodies that recognize xxx" in your Materials and Methods section. In the text, you could write the target proteins for each IP. To me, that's more formal than 'ChIP'd'
 

ADD COMMENTlink written 5.6 years ago by michael.law2610

yeah, I was just wandering, some papers have it in (but there are no rules seems). Thanks for the input.

ADD REPLYlink written 5.6 years ago by Sukhi Singh10k
1
gravatar for Ying W
5.6 years ago by
Ying W4.0k
South San Francisco, CA
Ying W4.0k wrote:

ChIP'd is not formal. If you cannot expect your audience to know what ChIP is, protein pull down is a more general term to use and you would probably need to define it first with something like "we pulled down DNA bound by protein X using chromatin immunoprecipitation (ChIP) followed by ...".

It is common to write "we performed ChIP with antibody against X to measure Y". Often times you just reference another paper in methods/protocol on how ChIP was performed. Make sure you include catalog number of antibody that you used.

ADD COMMENTlink written 5.6 years ago by Ying W4.0k

Thanks Ying, thats what I did exactly though I haven't mentioned the anitbody details but I referred another lab member's thesis who carried out all the experiments and has detailed that.

ADD REPLYlink written 5.6 years ago by Sukhi Singh10k
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