Question: Why the Allele frequency changed when using different variant calling tools?
0
gravatar for murali
4.6 years ago by
murali100
Germany
murali100 wrote:

I am doing variant calling for a sample using SAMtools, GATK, Platypus, Freebayes. I observed the allele frequency generated by different tools varies for the some of variants. The allele frequency has to be same , but why it is changing?

ADD COMMENTlink modified 4.6 years ago by Pierre Lindenbaum127k • written 4.6 years ago by murali100
4
gravatar for Pierre Lindenbaum
4.6 years ago by
France/Nantes/Institut du Thorax - INSERM UMR1087
Pierre Lindenbaum127k wrote:

"allele frequency has to be same" : that is not true if the  min/max criteria for "base quality"/"read quality"/"read flags", etc...  are not the same

ADD COMMENTlink written 4.6 years ago by Pierre Lindenbaum127k
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