BLAST step of GO enrichment analyses
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6.1 years ago
Ginsea Chen ▴ 130

Dear all!

I used nr database which download from NCBI as query database in BLAST step of GO analyses, and then I loaded this result file into Blast2go basic software. However, lots of sequences had no hits. I don't know what happened and whether this bad blast will influence my GO results or not ? 

 

BLAST; GO • 1.3k views
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6.1 years ago

There are different strategies that blast can be run with that will differ in sensitivity and speed: megablast , discontiguos, megablast, blastn, blastn-short etc  in addition you can also set different alignment parameters.

Depending on the situation you may want to run blast with more sensitive strategies.

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