Question: BLAST step of GO enrichment analyses
gravatar for Ginsea Chen
3.5 years ago by
Ginsea Chen120
Chinese Academy of Tropical Agricultural Sciences, Danzhou, China
Ginsea Chen120 wrote:

Dear all!

I used nr database which download from NCBI as query database in BLAST step of GO analyses, and then I loaded this result file into Blast2go basic software. However, lots of sequences had no hits. I don't know what happened and whether this bad blast will influence my GO results or not ? 


blast; go • 859 views
ADD COMMENTlink modified 3.5 years ago by Istvan Albert ♦♦ 79k • written 3.5 years ago by Ginsea Chen120
gravatar for Istvan Albert
3.5 years ago by
Istvan Albert ♦♦ 79k
University Park, USA
Istvan Albert ♦♦ 79k wrote:

There are different strategies that blast can be run with that will differ in sensitivity and speed: megablast , discontiguos, megablast, blastn, blastn-short etc  in addition you can also set different alignment parameters.

Depending on the situation you may want to run blast with more sensitive strategies.

ADD COMMENTlink written 3.5 years ago by Istvan Albert ♦♦ 79k
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