Question: How to normalize RNAseq raw read counts with out control?
gravatar for ifudontmind_plzz
4.6 years ago by
ifudontmind_plzz150 wrote:


I have few RNAseq data (raw read counts) from mouse  tumor samples. I dont have any controls or biological replicates. How can i normalize these type of data set?






rna-seq R • 1.5k views
ADD COMMENTlink written 4.6 years ago by ifudontmind_plzz150

You could median normalize every row across samples. That way you can still identify extreme changes.

ADD REPLYlink written 4.6 years ago by Matt Shirley9.3k
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