Question: Trim barcode 3'
0
gravatar for pingEde
3.2 years ago by
pingEde20
European Union
pingEde20 wrote:

Hello,

I have an question: I have to trim barcode at 3' and the sequence is reversed, cutadapt is able to remove this sequence or I have to do also in the file barcode.fasta the reverse sequence?

Could you suggest a good tool to remove a list of primer in the read?

thank you very much for your help


 

cutadapt trim primer barcode • 1.0k views
ADD COMMENTlink modified 3.2 years ago by SES8.1k • written 3.2 years ago by pingEde20

What do you mean by "reversed"? 

5' - ACCGGGTTT - 3'

Which gaves: TTTGGGCCA, 
or with complementary reversion TGGCCCAAA? 
 

ADD REPLYlink written 3.2 years ago by glihm580

Complementary reversion.

Thank you for your help!
 

ADD REPLYlink written 3.2 years ago by pingEde20
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