Trim barcode 3'
0
0
Entering edit mode
6.1 years ago
pingEde ▴ 40

Hello,

I have an question: I have to trim barcode at 3' and the sequence is reversed, cutadapt is able to remove this sequence or I have to do also in the file barcode.fasta the reverse sequence?

Could you suggest a good tool to remove a list of primer in the read?

thank you very much for your help


 

cutadapt barcode primer trim • 1.7k views
ADD COMMENT
0
Entering edit mode

What do you mean by "reversed"?

5' - ACCGGGTTT - 3'

Which gaves: TTTGGGCCA,

or with complementary reversion TGGCCCAAA?

ADD REPLY
0
Entering edit mode

Complementary reversion.

Thank you for your help!

ADD REPLY

Login before adding your answer.

Traffic: 3026 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6