I saw the announcement directing participants to carefully read the instructions about applying for Amazon cloud grants---however, it was not clear where these instructions would be located. I am inferring that cloud storage will be needed for the hands-on exercise, and that Amazon will be offering some storage on a limited basis.
I'm trying to do quality control for raw data for a genomics class, here is the fastqc report for Read1!
my questions are:
in this case should I do trimming or masking?
if trimming, should I trim the position 14-15 and 54-55 ? I could understand the logic behind trimming the 3' end but how I can trim in the middle of the read? Im sorry if my question quite basic but Im really suffering in understanding this concept and if u guys kindly provide me with any reference to read I would highly appreciate ur kindness.