excluding mammalia from Blastp

Question

[celibertofrancesca]

Hi, I'm a biology student and I'm using Blast for the first time in my life. I have make a similarity searching between my query, human cox4, and mammals genomes. If I select in Blast parameters, "organism: mammalia" and I also select "exclude", I won't have mammals entries?

Answer 1

[Andrzej Zielezinski]

Yes, by doing that you won't have mammalian entries. BLAST will search everything but mammals.