I got Illumina mate pair data. The insert size distribution is as follows:
1. What do you think about this distribution in general?
2. This library was done without targeting for a certain insert size length. What is the variation of the insert size if you enrich for a certain size? Do have any source or an example?
3. I would like to use this data together with Illumina PE. For example using spades. We want to assemble Plasmids from 90kb to 150kb. Do you think this library is suitable? Would you target a specific size? What techniques do you use?