Question: Question about FIELD information in samtools
0
gravatar for mangfu100
3.8 years ago by
mangfu100710
Korea, Republic Of
mangfu100710 wrote:

Hi all.

I am using the samtools to annotate my variants information and also am trying to use the I16 Field provided by samtools.

you can see its manual at http://samtools.sourceforge.net/mpileup.shtml

There are a total of 16 independent values in I16 field and first one is "#reference Q13 bases on the forward strand".

I almost understood the meaning of definition above statement but ,in order to better understand,  I posted here!

I think that definition of Q13 is quality score 13. 
Or is that indicating the number of reads that are more than quality score of 13?

I am confused.

On top of that, what is the tail distance? Although I tried to find detailed information about the I16 field, but I failed.

Thanks.

sequencing sequence next-gen • 860 views
ADD COMMENTlink modified 3.8 years ago by Istvan Albert ♦♦ 81k • written 3.8 years ago by mangfu100710
1

Q13 is a quality score of at least 13 (i.e., >=13).

Perhaps "tail distance" is the distance to the end of the read, but that's just a guess (thus why this is a comment).

ADD REPLYlink written 3.8 years ago by Devon Ryan91k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1565 users visited in the last hour