I am using the samtools to annotate my variants information and also am trying to use the I16 Field provided by samtools.
you can see its manual at http://samtools.sourceforge.net/mpileup.shtml
There are a total of 16 independent values in I16 field and first one is "#reference Q13 bases on the forward strand".
I almost understood the meaning of definition above statement but ,in order to better understand, I posted here!
I think that definition of Q13 is quality score 13.
Or is that indicating the number of reads that are more than quality score of 13?
I am confused.
On top of that, what is the tail distance? Although I tried to find detailed information about the I16 field, but I failed.