Question: Primers Within Reads
1
gravatar for Sam
7.9 years ago by
Sam10
United States
Sam10 wrote:

How can I remove primers from reads. Note: reads have small letters attached on both ends,are those primers?

read • 1.4k views
ADD COMMENTlink written 7.9 years ago by Sam10
2

You will need to provide a lot more details to get an informative answer to your question. What sequencing technology are you using? Which formats are your reads in? Were your reads pre-processed?

ADD REPLYlink written 7.9 years ago by Eric Fournier1.4k

I think you are talking about adapter sequence, and possibly you are using 454? We shouldn't have to guess though, so I recommend you tell us all the facts. Just in case, adapter sequences will be clipped automatically when converting sff to fasta using the gs* tools coming with the roche sequencer.

ADD REPLYlink written 7.9 years ago by Michael Dondrup47k
1
gravatar for Istvan Albert
7.9 years ago by
Istvan Albert ♦♦ 81k
University Park, USA
Istvan Albert ♦♦ 81k wrote:

For data in FASTQ format the fastx toolkit offers some options. If your data is in other formats please edit your question and specify what you need.

ADD COMMENTlink written 7.9 years ago by Istvan Albert ♦♦ 81k
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