Question: Single-cell RNA-seq : for what questions ? and what are the classical pipelines ?
2
gravatar for schmilblik
4.7 years ago by
schmilblik20
France
schmilblik20 wrote:

Hello stars ! 

I'm trying to find out a little bit about single cell RNA-seq and i want to know in which context ( the major problems that this technique can answer )  and what are the pipelines used in general when we have both classical RNA-seq AND single-cell RNA-seq data ??  

Thanks in advance ,

rna-seq • 2.0k views
ADD COMMENTlink modified 4.7 years ago by Shicheng Guo8.3k • written 4.7 years ago by schmilblik20
1
gravatar for vivekbhr
4.7 years ago by
vivekbhr550
Germany
vivekbhr550 wrote:

Hi

This recent review might be useful to you..

ADD COMMENTlink modified 7 months ago by RamRS27k • written 4.7 years ago by vivekbhr550
1
gravatar for Shicheng Guo
4.7 years ago by
Shicheng Guo8.3k
Shicheng Guo8.3k wrote:
  • STAR: ultrafast universal RNA-seq aligner
  • TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions
  • Cufflinks: Transcriptome assembly and differential expression analysis for RNA-Seq.
  • Trinity: a novel method for the efficient and robust de novo reconstruction of transcriptomes from RNA-Seq data
  • SpliceMap: SpliceMap is a de novo splice junction discovery tool
  • Degust: User friendly RNA-seq differential expression analysis with Degust
ADD COMMENTlink modified 7 months ago by RamRS27k • written 4.7 years ago by Shicheng Guo8.3k
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