Question: Single-cell RNA-seq : for what questions ? and what are the classical pipelines ?
2
gravatar for schmilblik
3.4 years ago by
schmilblik20
France
schmilblik20 wrote:

Hello stars ! 

I'm trying to find out a little bit about single cell RNA-seq and i want to know in which context ( the major problems that this technique can answer )  and what are the pipelines used in general when we have both classical RNA-seq AND single-cell RNA-seq data ??  

Thanks in advance ,

rna-seq • 1.5k views
ADD COMMENTlink modified 3.4 years ago by Shicheng Guo7.4k • written 3.4 years ago by schmilblik20
1
gravatar for vivekbhr
3.4 years ago by
vivekbhr510
Germany
vivekbhr510 wrote:

Hi

This recent review might be useful to you.. 

ADD COMMENTlink written 3.4 years ago by vivekbhr510
1
gravatar for Shicheng Guo
3.4 years ago by
Shicheng Guo7.4k
Shicheng Guo7.4k wrote:

STAR: ultrafast universal RNA-seq aligner

TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions

Cufflinks: Transcriptome assembly and differential expression analysis for RNA-Seq.

Trinity: a novel method for the efficient and robust de novo reconstruction of transcriptomes from RNA-Seq data

SpliceMap: SpliceMap is a de novo splice junction discovery tool

Degust: User friendly RNA-seq differential expression analysis with Degust

 

 

ADD COMMENTlink modified 3.4 years ago • written 3.4 years ago by Shicheng Guo7.4k
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