So, currently, I have 1 pair of normal-cancer data from RNA-seq. I already generated gene level read counts with Salmon. What I want to do is to check for a certain gene whether it is up or down regulated. So, is there any problem with just 1 pair of sample? What tools will be suitable for 1 pair sample like this (DESeq2, limma, edgeR,etc)?
Thank you for your answers and suggestions.