I've been asked to convert some Phred64 fastq files to Phred33 fastq
How can I perform this ? Any recommended perl script?
The following pipeline works pretty well. Thanks Frank:
1, install fastq_phred_convert from github 2, export PERL5LIB=$PERL5LIB:/home/user/fastq_phred_convert/ 3, copy fastq_33_to_64.pl and fastq_63_to_33.pl to /usr/bin 4, use them as the traditional command: perl /usr/bin fastq_63_to_33.pl *input.fastq