Question: Psi-BLAST error : During COG analysis
gravatar for Mohit
4.2 years ago by
Mohit0 wrote:


I am trying to Run COG software

during the Blast results I am getting an error and program stops.. Can any one tell me why this error is :??


"   BLAST engine error: PSI-BLAST only accepts one protein sequence as query    "

Please guide regarding!

My annotation file contains as many as 5140 sequences. upto 1123  sequences it worked and after that It gave this error!
Is it the problem of Server memory?

Thank you!



cog blast psi-blast • 1.2k views
ADD COMMENTlink modified 4.2 years ago • written 4.2 years ago by Mohit0

Have you checked sequence #1123 +/- 1 to see if you can spot something obviously wrong?

ADD REPLYlink written 4.2 years ago by genomax77k

NO, I haven't, I'll try it too!

ADD REPLYlink written 4.2 years ago by Mohit0

This error was encountered by writing perl script which extract a sequence from a annotation file and performing psi-blast. This is how it was done for rest of sequences too.


ADD REPLYlink modified 7 weeks ago by RamRS25k • written 4.2 years ago by Mohit0
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