Question: Psi-BLAST error : During COG analysis
0
gravatar for Mohit
3.4 years ago by
Mohit0
India
Mohit0 wrote:

Hello,

I am trying to Run COG software

during the Blast results I am getting an error and program stops.. Can any one tell me why this error is :??

 

"   BLAST engine error: PSI-BLAST only accepts one protein sequence as query    "

Please guide regarding!

My annotation file contains as many as 5140 sequences. upto 1123  sequences it worked and after that It gave this error!
Is it the problem of Server memory?

Thank you!

Best,

Mohit

cog blast psi-blast • 1.0k views
ADD COMMENTlink modified 3.4 years ago • written 3.4 years ago by Mohit0

Have you checked sequence #1123 +/- 1 to see if you can spot something obviously wrong?

ADD REPLYlink written 3.4 years ago by genomax65k
0
gravatar for Mohit
3.4 years ago by
Mohit0
India
Mohit0 wrote:

NO, I haven't, I'll try it too!

ADD COMMENTlink written 3.4 years ago by Mohit0
0
gravatar for Mohit
3.4 years ago by
Mohit0
India
Mohit0 wrote:

This error was encountered by writing perl script which extract a sequence from a annotation file and performing psi-blast. This is how it was done for rest of sequences too.

 

Best,

Mohit 

 

ADD COMMENTlink written 3.4 years ago by Mohit0
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 857 users visited in the last hour