Question: (Closed) minimum nucleotide in a cluster of the illumina sequencing to get stable signals
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gravatar for Shicheng Guo
4.5 years ago by
Shicheng Guo8.2k
Shicheng Guo8.2k wrote:

Hi All, 

minimum nucleotide in a cluster of the illumina sequencing to get stable signals? I mean, one template will form a cluster by bridge-duplication. I wonder what's the minimum nucleotide or duplication NGS need to get stable image information to provided A,T,C,G?

 

Best regards, 

 

 

 

illumina cluster • 712 views
ADD COMMENTlink written 4.5 years ago by Shicheng Guo8.2k

Hello Shicheng Guo!

Sorry, this isn't a bioinformatics question. But you may find this link of interest: http://molbiol.ru/wiki/Clonal_amplification

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

ADD REPLYlink modified 4.5 years ago • written 4.5 years ago by Daniel Swan13k

Hi Daniel, Thank you so much for your tips. I have got the answer from the link you shared with me. "In contrast to normal PCR all solutions are used only for one cycle and then go to trash. It helps to keep low background. Otherwise occasionally detached DNA molecule could have a chance to hybridize in another place and start a new cluster there. Surface PCR has lower efficiency if compare with PCR in solution. 35 cycles result in ~1000 copies of the original molecule (in ideal PCR 35 cycles should give ~1010x amplification). The possible reasons of lower efficiency"

ADD REPLYlink written 4.5 years ago by Shicheng Guo8.2k
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