I am doing some real time pcrs, at each run with set of genes of interest and one housekeeping gene.
I would like to compare results of all this runs, so I could know relative expression levels of all my genes.
And thats when the problem comes. I have read articles about ∆∆CT method, which, according to my knowledge, compares genes of interests and housekeeping genes in both treated and untreated tissue.
I guess that my case is different - I have only one tissue (untreated) and I want to compare expressions of many genes, which unfortunately forces me to do many runs.
How can I do such comparision? We lead passionate discussions in our lab, so I will be really thankfull to hear your opininon! Thanks a lot :-)