5.8 years ago by
Santiago de Compostela, Spain
FastQC is just an informative tool, very useful in my opinion to evaluate how the lab sequencing jobs have performed before going into mapping. but as any informative tool, it just tells you what's going on down there, and of course you are the one ultimately having to curate those reads in case you need to. but you'll have to know that there are things that may be curated, and there are others that you won't be able to do anything with them.
since the available tests performed by FastQC are multiple, there isn't a particular to fix the reported errors if any. things like the "per base sequence quality" for instance may be solved by simple sequence trimming if the reported error is that the last 3 bases are always wrong, so they should always be removed from posterior analysis. other error reports may be also solvable by simple scripting, but most of them, such as "per sequence GC contect", are purely descriptive, and in case they fail to pass the FastQC thresholds there's nothing you can do about them but to repeat the lab sequencing if wanted.