Assembling Another Genome based on a Reference
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5.8 years ago
' ▴ 290

I have a bacteria sample, and I have done genome assembly and now have its original sequence. Since the bacteria sample has come from the soil, it contained two closely related bacterias. How can I use the reference genome that I now have to assemble the "other" genome? Which programs can be helpful?

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5.7 years ago
' ▴ 290

Here's what I did: I mapped my initial reads back to the contigs generated by Velvet (using BWA), then I had a look at it using Tablet. Then finally I used the SAM file generated by BWA and the contigs file I had (as reference) and the Columbus package of Velvet to generated a "hopefully" related sequence to my bacteria. I just have to mention that this task is not really biologically sound and was just a warm-up exercise I got myself involved in!

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You probably want to change "mapped" to "aligned" :-) ref: A: Alignment and mapping

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