I am currently dealing with some fasta files using fastools to digest the sequence by MboI restriction enzyme. The command I used is as follow fastools -digest -rest MboI -in A.fasta -out REA.fasta This enzyme cuts GATC sites, but the output also contain the complementary strand information which starts with CTAG. Therefore, I wanna ask whether it is possible to let the program only cut GATC instead of cutting CTAG? Or is there any other program to cut the DNA sequence in fasta file based on restriction site? Thanks!