For an experiment I am using tumour DNA and commercially available control DNA.
For the result of my aCGH, would it possible to use a different ratio of tumour DNA vs control DNA? E.g. tumour:control = 2:1 or visa versa tumour:control = 1:2
For example, if I were to use 500ng tumour DNA and 800ng control DNA for an array CGH, would this influence the result? Intuitively I would think using more control DNA than tumour DNA would bring down any amplifications and increase deletions... Am I correct? And if so, could this be corrected in the analysis?