MIcroarray data analysis?
1
0
Entering edit mode
8.1 years ago

Hi Can we combine or correlate the results of NGS and Microarray data? In the results of GEO2R some of the value are missing? How i treat that probe id?

next-gen • 1.8k views
ADD COMMENT
0
Entering edit mode

You can use missing value imputation. impute package form bioconductor

ADD REPLY
0
Entering edit mode
8.1 years ago

You should rename your question and/or ask two separate questions.

I've found that it is possible to compare microarray and RNA-Seq gene expression values by comparing the expression of one set of genes relative to another set of genes (in the links below, TCGA is RNA-Seq while all other datasets are microarrays).

https://peerj.com/articles/159/#table-3 https://peerj.com/articles/159/#fig-3

In general, the absolute values won't be comparable (and I wouldn't recommend doing anything like quantile normalization to make them similar), and the fold-change values for individual genes will probably also be different (although you could do independent analysis and look for overlapping genes).

ADD COMMENT

Login before adding your answer.

Traffic: 1574 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6