I have a bacterial genome and a predicted proteome from the same genome. How do I generate a Genbank (without submitting it)?

Question

[Selenocysteine]

Hi all,

I have a set a bacterial genome (fasta format) and a multifasta file with all the predicted proteins from the same genome, translated using bioperl.

In order to use a particular software to analyze these sequences, I need to have a genbank format file of the genome, shaped as:

  LOCUS       SCU49845     5028 bp    DNA             PLN       21-JUN-1999
DEFINITION  Saccharomyces cerevisiae TCP1-beta gene, partial cds, and Axl2p
            (AXL2) and Rev7p (REV7) genes, complete cds.
ACCESSION   U49845
VERSION     U49845.1  GI:1293613
KEYWORDS    .
SOURCE      Saccharomyces cerevisiae (baker's yeast)
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes;
            Saccharomycetales; Saccharomycetaceae; Saccharomyces.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Torpey,L.E., Gibbs,P.E., Nelson,J. and Lawrence,C.W.
  TITLE     Cloning and sequence of REV7, a gene whose function is required for
            DNA damage-induced mutagenesis in Saccharomyces cerevisiae
  JOURNAL   Yeast 10 (11), 1503-1509 (1994)
  PUBMED    7871890
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   Roemer,T., Madden,K., Chang,J. and Snyder,M.
  TITLE     Selection of axial growth sites in yeast requires Axl2p, a novel
            plasma membrane glycoprotein
  JOURNAL   Genes Dev. 10 (7), 777-793 (1996)
  PUBMED    8846915
REFERENCE   3  (bases 1 to 5028)
  AUTHORS   Roemer,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-1996) Terry Roemer, Biology, Yale University, New
            Haven, CT, USA
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Saccharomyces cerevisiae"
                     /db_xref="taxon:4932"
                     /chromosome="IX"
                     /map="9"
     CDS             <1..206
                     /codon_start=3
                     /product="TCP1-beta"
                     /protein_id="AAA98665.1"
                     /db_xref="GI:1293614"
                     /translation="SSIYNGISTSGLDLNNGTIADMRQLGIVESYKLKRAVVSSASEA
                     AEVLLRVDNIIRARPRTANRQHM"
     gene            687..3158
                     /gene="AXL2"
     CDS             687..3158
                     /gene="AXL2"
                     /note="plasma membrane glycoprotein"
                     /codon_start=1
                     /function="required for axial budding pattern of S.
                     cerevisiae"
                     /product="Axl2p"
                     /protein_id="AAA98666.1"
                     /db_xref="GI:1293615"
                     /translation="MTQLQISLLLTATISLLHLVVATPYEAYPIGKQYPPVARVNESF
                     TFQISNDTYKSSVDKTAQITYNCFDLPSWLSFDSSSRTFSGEPSSDLLSDANTTLYFN
                     VILEGTDSADSTSLNNTYQFVVTNRPSISLSSDFNLLALLKNYGYTNGKNALKLDPNE
                     VFNVTFDRSMFTNEESIVSYYGRSQLYNAPLPNWLFFDSGELKFTGTAPVINSAIAPE
                     TSYSFVIIATDIEGFSAVEVEFELVIGAHQLTTSIQNSLIINVTDTGNVSYDLPLNYV
                     YLDDDPISSDKLGSINLLDAPDWVALDNATISGSVPDELLGKNSNPANFSVSIYDTYG
                     DVIYFNFEVVSTTDLFAISSLPNINATRGEWFSYYFLPSQFTDYVNTNVSLEFTNSSQ
                     DHDWVKFQSSNLTLAGEVPKNFDKLSLGLKANQGSQSQELYFNIIGMDSKITHSNHSA
                     NATSTRSSHHSTSTSSYTSSTYTAKISSTSAAATSSAPAALPAANKTSSHNKKAVAIA
                     CGVAIPLGVILVALICFLIFWRRRRENPDDENLPHAISGPDLNNPANKPNQENATPLN
                     NPFDDDASSYDDTSIARRLAALNTLKLDNHSATESDISSVDEKRDSLSGMNTYNDQFQ
                     SQSKEELLAKPPVQPPESPFFDPQNRSSSVYMDSEPAVNKSWRYTGNLSPVSDIVRDS
                     YGSQKTVDTEKLFDLEAPEKEKRTSRDVTMSSLDPWNSNISPSPVRKSVTPSPYNVTK
                     HRNRHLQNIQDSQSGKNGITPTTMSTSSSDDFVPVKDGENFCWVHSMEPDRRPSKKRL
                     VDFSNKSNVNVGQVKDIHGRIPEML"

Is there any intelligent and quick way to do this using external software / Python / R? I reckon I can go on and manually parse the file but I don't think it is the smartest way to proceed...

Ah, and I don't want to submit the sequences to NCBI so I think I can't use the software they provide for their submissions, as it seems to be that it requires to submit the file. Thanks a lot!

[dago]

Did you think to re-annotated your genomes with a pipeline that produces gbk (e.g. PROKKA, RAST)?

[Selenocysteine]

Thanks :) I had to use annotations previously made by someone else, in the end I just wrote a manual parser using Python.