Capillary sequencing data analysis
1
0
Entering edit mode
5.8 years ago

Hello, I m looking for a tool which can trim the AB1 file and gives me the fasta and quality file so i can go for the further analysis.

P.S. i used the phred but it dnt give the freedom to setup the quality values . even if any one knows how to do that then thanks in advance

sequencing genomics ab1 gene • 1.4k views
ADD COMMENT
1
Entering edit mode
5.8 years ago
Amitm ★ 2.1k

hi, Lasergene's DNAStar had the capability of working with capillary seq. data when I was working on such data. Check for details on website.

ADD COMMENT
0
Entering edit mode

DNAStar is not free software. Just so you don't raise @kapil's hopes.
@kapil: DNABaser claims to be freeware and can edit ab1 files (not sure if the lite version has that capability). Use at your discretion.

ADD REPLY
0
Entering edit mode

hi, apologies. Advertising paid software was not the intention. Thanks though. I am not aware of any free s/w with this feature.

ADD REPLY
0
Entering edit mode

Generally when people are asking about software they are looking for free options that was the reason I added the clarification for DNAStar. There is no restriction about mentioning commercial software on Biostars as far as I know.

ADD REPLY

Login before adding your answer.

Traffic: 3157 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6