Question: Is there any reason to analyze plus/minus strands separately in bisulfite seq/methylation data?
1
gravatar for biohack92
4.7 years ago by
biohack92150
United States
biohack92150 wrote:

I have bisulfite sequencing data that consists of read count data. The data is split based on strand orientation (plus & minus). What sort of questions can be answered by analyzing the plus/minus strand separately?

ADD COMMENTlink modified 4.7 years ago by Antonio R. Franco4.5k • written 4.7 years ago by biohack92150
2
gravatar for Devon Ryan
4.7 years ago by
Devon Ryan98k
Freiburg, Germany
Devon Ryan98k wrote:

The only normal reasons are to test for asymmetric methylation and to see if one particular base in a motif is methylated or not (this can alter factor binding).

ADD COMMENTlink written 4.7 years ago by Devon Ryan98k
1
gravatar for Antonio R. Franco
4.7 years ago by
Spain. Universidad de Córdoba
Antonio R. Franco4.5k wrote:

In plants, it has been described a non symmetrical methylation in domains other than CpG, such as CHH or CHG See this paper

ADD COMMENTlink written 4.7 years ago by Antonio R. Franco4.5k
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