Question: Qpcr Gene Expression Fold Change Analysis : Which Simple Statistical Test Would Fit!!!!
1
gravatar for Sukhi Singh
8.8 years ago by
Sukhi Singh10k
Netherlands
Sukhi Singh10k wrote:

Hi, I have 23 different targeting clones (divided in 3 groups) in which a same gene is expressed and I have the fold change values of these genes (calculated from CT values against a house keeping gene as control).

So, I would like to use a test, where I can show the difference among the groups is significant or not. What could I use (t-test paired - unpaired, dependent or something else ??)

I use R so a specialized package I wont mine, I know there are packages like HTqPCR,NormqPCR but they go from start (CT values) and I already have the fold change values and the standard error, so a simple test would be good.

Thanks

gene R statistics • 4.5k views
ADD COMMENTlink written 8.8 years ago by Sukhi Singh10k

Given you got more than two groups, Anova or non-parametric alternatives could be it. Is that enough detail?

ADD REPLYlink written 8.8 years ago by Michael Dondrup48k

I think, I should go for Anova because of 3 groups or Kruskal–Wallis. Thanks

ADD REPLYlink written 8.8 years ago by Sukhi Singh10k
0
gravatar for Julien Textoris
8.8 years ago by
Marseille, France
Julien Textoris430 wrote:

You may have a look at this amazing website on R and stats and the book that was written R in action. It has helped me several times to choose which test is best.

ADD COMMENTlink written 8.8 years ago by Julien Textoris430

Thanks Julien, this looks like a nice R reference.

ADD REPLYlink written 8.8 years ago by Sukhi Singh10k
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