Bowtie2 result analysis
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4.8 years ago

Hi all, i am perform local alignment with bowtie2. the output is: -

17267759 reads; of these:
17267759 (100.00%) were unpaired; of these:
129533 (0.75%) aligned 0 times
8205103 (42.52%) aligned exactly 1 time
8933123(51.73%) aligned >1 times
99.25% overall alignment rate

i don't know it is right or not if it is right then how to analyse this, please help me. thank you

alignment • 1.6k views
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2
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4.8 years ago

Without any context all anyone can say is that you got a nice alignment rate. Have a look at the alignments in IGV or something like that and see if they seem reasonable.

Edit: There's nothing to really analyse there. You have some summary metrics with nothing to compare them to.

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4.8 years ago
Sej Modha 4.8k

Could you please provide more information as to why you think this results is not right?

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hi.. This Alignment are not classified in concordant or disconcordont. so i think...is it wrong alignment?

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Were they paired end?

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no..i have clean reads which are download from lotus database http://202.127.146.141/.so i perform local alignment

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If you don't have paired-end data then there's no conception of "concordant" and "discordant" that could be quantified.

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you mean it is right local alignment?

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I'm sorry, "you mean it is right local alignment" can have too many meanings. If you mean, "So, does the output I posted from bowtie2 indicate that it likely ran correctly and to completion?", then yes.

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