VCF - Annotation

Question

[manojkumarbioinfo]

Hi,

I would like to annotate my vcf file.I have mapped my Raw data of Pseudomonas_aeruginosa using BWA and their variant were called using samtools mpileup. now i wish to annotate my vcf file to find out whether any one of my variants found in the coding reagion. i tried different tools for annotation but many dont have the data base regrinding Pseudomonas_aeruginosa for annotation purpose. if any one have any idea for annotating the vcf file kindly help me

Answer 1

[Emily]

Have you tried the Ensembl VEP?

[manojkumarbioinfo]

@Emily Thank you for your help :)