Entering edit mode
7.9 years ago
zizigolu
★
4.3k
Hi,
I received my qPCR results from lab.
for each gene i have a log2 fold change and a standard deviation
I have 4 treatment versus control
for example for gene AT1G18970
log2FC sd
4h 1.21810725 0.100993
8h 3.3357405 0.19981
24h 0.2808515 0.198745
48h 0.8401785 0.0983347
may someone tell me please for report in paper the expression of this gene is confirmed or not??? because I can't get how I detect based on sd and log2FC whether this genes deferentially expressed or not.
thank you
@Angel what is this log2FC ? if you have 4 treatments and controls for it, you should also say how many replicate you have , and I assume then you can have 4 logFC and not only 1, But looking at your example, I found that it is a synthesis experiments, again if this is your data and not portion of it, would not be possible to tell only based on sd
Thank you I heated Arabidopsis seedlings for 4, 8, 24 and 48 hours and compared them with un-heated controls in this time points. I did not have replication in my experiment. I did not do qPCR myself. I only selected 10 genes randomly from heat responding genes and asked a lab to perform qPCR. they sent a file contains log2FC and standard deviation for each gene in compared to match control for example heated 4 hours-control 4 hours. now I don't know what to say, whether this genes were deferentially expressed or not.
If that's the case then you can't detect differential expression. You would need replicates for that, regardless of the experiment done.