Can anyone comment on what the outcomes would be for using a tissue sample (human PDX tumour grown in a mouse) that likely contains both human and mouse cells for a microarray (to gauge RNA expression)?
That is, would mouse RNAs be able to hybridize? I am not familiar with the chip selection process for a microarray (I have never done one before) but I assume I would choose a chip whose probes were human sequences? I am interested in expression of RNAs from the human tumour cells collected after this PDX mouse model was treated with a drug.
I am not clear on how much similarity there would be between human and mouse RNAs and thus whether I need to worry about mouse RNAs binding to the (human) probes or not?
Of course, all of this could be avoiding by depleting the sample of mouse cells before proceeding with the array, but unfortunately that will not be possible for me at this time.
Looking for any insight in this area.