Hi, I am trying to generate mouse geneome indexes with STAR to align my RNAseq data. I downloaded the mm10 genome in tar.gz format (Mus_musculus_UCSC_mm10.tar.gz). Do I have to gunzip this first before trying to index the genome? Thank you!
I had the same problem, for me it was becuase I was using a database of all human viruses as opposed to a genome. This had too many contigs, I read in a google post to increase the genomeChrBinNbits 14, that did it.