Question: RNA-seq results comparison possible or not?
0
gravatar for mahnazkiani
2.8 years ago by
mahnazkiani40
United States
mahnazkiani40 wrote:

I have 2 sets of data for my plant, the first set included 3 biological reps from plants that grown in 3 different times and got the samples from 2 parts of the stem to compare cell wall genes expression, my second set of plants grown in different light condition but still have 2 biological reps. Now I want to see does light affect the cell wall gene expression or not? Can I do this comparison between these 2 sets of data? How about the batch effects? The problem is that I didn't include any control in second sets of plants and I don't know how I can find about the batch effects or is it even possible to adjust the batch effects?

Thanks

rna-seq • 831 views
ADD COMMENTlink modified 2.8 years ago by Biostar ♦♦ 20 • written 2.8 years ago by mahnazkiani40
1

If your 2 experiments are performed in 2 separate batches, it doesn't make sense to include the batch in your model.

ADD REPLYlink written 2.8 years ago by Benn6.6k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 918 users visited in the last hour