RNA-seq results comparison possible or not?
0
0
Entering edit mode
7.8 years ago
mahnazkiani ▴ 60

I have 2 sets of data for my plant, the first set included 3 biological reps from plants that grown in 3 different times and got the samples from 2 parts of the stem to compare cell wall genes expression, my second set of plants grown in different light condition but still have 2 biological reps. Now I want to see does light affect the cell wall gene expression or not? Can I do this comparison between these 2 sets of data? How about the batch effects? The problem is that I didn't include any control in second sets of plants and I don't know how I can find about the batch effects or is it even possible to adjust the batch effects?

Thanks

RNA-Seq • 1.5k views
ADD COMMENT
1
Entering edit mode

If your 2 experiments are performed in 2 separate batches, it doesn't make sense to include the batch in your model.

ADD REPLY

Login before adding your answer.

Traffic: 2771 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6