Hi everybody, I have had a de novo assembly, and now I want to get the annotation, I have the predicted genes from geneMarks, but, somebody knows what I have to do for validate that genes? How can I do that? from coverage for the predicted genes? And, after that, does exist any program (linux command line) for creating gff file? or I must mix output from blast format 6 with gff from gene prediction? Thanks a lot! for suggestions!
Thanks! it really helps me!