I have a very basic question (apology, but get confused) In calculation of fold change in gene expression data I am doing Treated - control samples and then use the formula (IF(R2>1,R2,-1/R2)) (assuming R has the results. So my questions are : 1. Do we do Treated - control or other way around. 2. Is it OK to use (IF(R2>1,R2,-1/R2) above as it will take care of down regulation.
Thanks Rob
This is real time PCR data Theoretically should we do Treatment - control or only Treatment / control is good
In the case of real time PCR data, the links provided by Natasha may prove to me more informative. Do you mean to subtract the signal for control from the signal from Treatment? I'm sure for qPCR there is also specific software available.
Please use tags accordingly to the content of your question, you listed RNA-seq but this isn't RNA-seq.