I'm busy with doing expression analysis of an RNA-seq project from a non-model organism by edgeR software. I previously did de novo transcriptome assembly by Trinity. I performed GO analysis by agrigo for up- and down-regulated genes, but I found that several GO terms of interest overrepresented in the both up- and down-regulated gene list. Could you please tell me if it's normal? how can we interpret such a result? any comments and suggestion would be highly appreciated.