how to get hESCs ChIP-seq data?
2
0
Entering edit mode
7.7 years ago

Dear Biostars,

I am working on to get all human embryonic stem cell (hESC) experiments ChIP-seq data from available databases like GEO and SRA databases. As a newbie I dont know how and where to start to complete this task. Can someone help in this regard. Thank you.

ChIP-Seq • 1.3k views
ADD COMMENT
1
Entering edit mode
7.7 years ago
EagleEye 7.5k

Term name ES-I3 (I guess these cells are undifferentiated)

Term ID EFO:0007076

Summary ES-I3 stem cell female embryo

Description The I3 stem cell line was derived from donated blastocysts by removal of the trophoblast layer and further culturing of the intact inner cell mass on mitotically inactivated mouse embryonic fibroblast (MEF) feeder cells. Following injection into SCID mice, the I3 line formed teratomas that contained tissues from all three embryonic germ layers. This line exhibits a normal XX karyotype. These cells were provided by Alex Meissner and maintained in mTeSR medium.

https://www.encodeproject.org/reference-epigenomes/ENCSR747ULU/

http://www.ncbi.nlm.nih.gov/geo/roadmap/epigenomics/?view=samples&sample=ES-I3%20cell%20line

ADD COMMENT
0
Entering edit mode
7.7 years ago
GenoMax 141k

This should get you started.

ADD COMMENT

Login before adding your answer.

Traffic: 2266 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6