what information is inclued in QNAME (Read ID) in sam file
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7.7 years ago
Joe ▴ 30

I can find this description online: QNAME: Query template NAME. Reads/segments having identical QNAME are regarded to come from the same template. A QNAME ‘*’ indicates the information is unavailable. In a SAM file, a read may occupy multiple alignment lines, when its alignment is chimeric or when multiple mappings are given.

The qname example is: M04402:7:000000000-ANGUV:1:2103:23029:8212

This is from a Miseq run, so what is the meaning of these number?

Thanks!!

bam sam qname • 8.1k views
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7.7 years ago
GenoMax 142k

If you are only interested in the meaning of that header (referred to as qname in SAM format) then check this link.

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Thanks a lot, that is exactly what I want.

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7.7 years ago
Sej Modha 5.3k

QNAME: Query template NAME. For further info visit samtools format specs

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