I have a ~30X PacBio dataset and ~40X Illumina dataset, besides of a mate-pair dataset. I am trying to assemble them (the expected genome size is around ~230Mb, it's a plant) in a server with 160Gb of RAM. However, I am having problems with ALLPATHS (lack of memory at the step of CorrectLongReads).
Is that possible to assemble my data in a cluster with 160Gb of RAM?
Is that possible with ALLPATHS?