Hi, I have a project using RNA-Seq but there are no replicates for each condition. I am wondering what method I should use for such a situation. Especially, how I should normalize the data? Apparently, I cannot use limma-voom pipeline. It seems I may be able to use DESeq2. But I am wondering if it's possible to directly compare TPM for each condition using Kallisto as the reads somehow normalized to TPM scale. I haven't seen such discussions using Kallisto before but I would like to know if you have any thoughts on this. Thank you.