Question: Mapping using multiple reference sequences
gravatar for Juan Ledesma
2.6 years ago by
United Kingdom
Juan Ledesma0 wrote:


I am using BWA to map my FASTQ files against two different reference sequences (V3-1 and V3-2) simultaneously on Galaxy to see the reads belonging to each sequence.

Once I've got the SAM file I would like to filter the mapped reads to generate two BAM files in order to get one file with the reads mapping V3-1 and another with those mapping V3-2.

Does any one know how I could filter these reads on Galaxy?

Many thanks


bwa sam bam • 1.1k views
ADD COMMENTlink modified 2.6 years ago • written 2.6 years ago by Juan Ledesma0

Thank you Brian, sounds interesting. I will try

ADD REPLYlink written 2.6 years ago by Juan Ledesma0

Please use ADD REPLY/ADD COMMENT when responding to existing posts to keep threads logically organized.

ADD REPLYlink written 2.6 years ago by genomax68k
gravatar for Brian Bushnell
2.6 years ago by
Walnut Creek, USA
Brian Bushnell16k wrote:

Unless you are strictly limited to using Galaxy, I suggest using BBSplit for this purpose. It maps to all references at once, and produces one output fastq per reference (assigning reads to the reference they match best, when they map to more than one with different mapping scores), which is more accurate for resolving ambiguity than mapping one at a time and trying to postprocess the sam files.

ADD COMMENTlink written 2.6 years ago by Brian Bushnell16k
gravatar for Devon Ryan
2.6 years ago by
Devon Ryan90k
Freiburg, Germany
Devon Ryan90k wrote:

That won't be very simple to do in Galaxy. The only route that I can advise is for you to use the python interactive environment, since pysam is probably already available in it. This would require you to know how to program in pysam, of course.

Having said that, you might want to post this question to the Galaxy site, where hopefully you'll get a simpler answer :)

ADD COMMENTlink written 2.6 years ago by Devon Ryan90k

Thanks for your comments Devon. I will try to learn how to do it with python but in the meantime I will post the question on the Galaxy site, just in case.

ADD REPLYlink written 2.6 years ago by Juan Ledesma0
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