Question: blast strand plus minus
0
gravatar for Picasa
3.4 years ago by
Picasa490
Picasa490 wrote:

Hi,

I have a doubt wit hthe blast output. So I have blast scaffolds to genes in order to do a multiple alignment.

Should I reversed-complement all my scaffolds that have been assigned to strand:

+ -
- +
- -

(First part is my query, second is the subject)

The idea is to get all the scaffolds to the same direction for multiple alignment.

strand blast • 1.3k views
ADD COMMENTlink modified 16 months ago by Biostar ♦♦ 20 • written 3.4 years ago by Picasa490

I’m not sure I fully understand, but BLAST will let you specify whether you want to retrieve matches that are on the plus, minus, or both strands.

ADD REPLYlink written 16 months ago by Joe16k
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