I am new to using mothur and in attempting to follow the MiSeq_SOP guide with my data on 18S rRNA v4 sequences I am stumped in the beginning. The guide assumes you want to make contigs from paired-end reads, but I would like to analyze my forward reads separately. How do I combine just my read 1 fastq files, retaining the sample name for all the sequences in each file of course, so that I can proceed with the following alignment and classification steps? I can't seem to find an explanation on how to do this anywhere.
Question: Analyzing only forward reads in mothur
13 months ago by
giderk • 0
giderk • 0 wrote:
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